木麻黄小枝基因组DNA的快速提取研究

Study on the Fast Extraction of Genome DNA from Fresh Twigs and Dry Twigs of Casuarina equisetifolia

提取介质中加入２５％ β巯基乙醇， 能有效去除次生物质对木麻黄基因组ＤＮＡ 提取的影响． 用新建方法所提ＤＮＡ 产率比用前人方法提的高出０８ 倍左右． 鉴定结果表明： 鲜小枝、干小枝的ＤＮＡ 样品皆为４８ｋｂ 左右， 适于限制性酶切和ＲＡＰＤ 反应； 同时发现同一株树的鲜小枝、干小枝，基因组ＤＮＡ

The extraction medium,which mixed with 2.5%β mercaptoethanol,can eliminate the secondary materials' effections to extracting gemone DNA from Casuarina equisetifolia effectively.The yields of genome DNA extract with the new method are 1 8 times as many as those of the DNA extracted with the former method.The result of characterization shows that the molecular weights of genome DNA from fresh twigs and dry twigs are 48kb,which are all suitable for digesting with restrictive enzyme and RAPD analysis.In addition, DNA templates extracted from fresh twigs and dry twigs produce the same RAPD result.