CdSe@ZnS量子点在果蝇及其幼虫体内的分布

Distribution of CdSe@ZnS QDs in adult drosophila and their stage-1 larva

用同步辐射微束X射线荧光(μ-SRXRF)技术结合激光扫描共聚焦显微成像(LSCM)技术研究了经CdSe@ZnS量子点口暴露的果蝇成虫及其子代一期幼虫体内的微区分布。μ-SRXRF分析表明,果蝇成虫摄取CdSe@ZnS量子点后,果蝇成虫胃肠道组织中有Cd、Zn和Se元素的富集,说明量子点经果蝇口服暴露后主要富集在肠道和生殖道部位。LSCM观察表明,CdSe@ZnS量子点暴露后成虫所产一期幼虫的生殖部位(精巢)有明显的荧光增强信号,表明量子点暴露能够经果蝇母体转运到子代。μ-SRXRF分析技术为纳米颗粒在活体内转运研究提供了很好的分析手段。

In this work,CdSe@ZnS quantum dots（QDs） distributed in the QDs-exposed adult drosophila and their stage-1 larva were characterized by synchrotron radiation micro X-ray fluorescence（μ-SRXRF） and laser scanning confocal microscope（LSCM）,respectively.The CdSe@ZnS QDs were exposed via the mouth to the adult drosophila.The μ-SRXRF elemental mapping of Cd,Se and Zn revealed that the elements were concentrated in the intestinal and reproductive system,indicating that QDs could accumulate in the intestinal and reproductive system.Obvious fluorescence signals were observed in the testis of stage-1 larva by LSCM technique.The results indicate the QDs via oral exposure could induce potential impact on placenta translocation and reproductive system.The results show that μ-SRXRF analysis is a novel method for the investigation of translocation of nanoparticle in the living organism.