丙酮酸钠液对烫伤休克大鼠肾血管通透性的影响

Study on the effect of pyruvate on the kidney vascular permealbility in rats with scalded injury

目的观察丙酮酸钠对烫伤休克大鼠肾通透性的影响。方法采用50%TBSAⅢ度烫伤大鼠模型,大鼠随机分为假烫组、烫伤组、氯化钠组和丙酮酸钠组,每组6只。应用伊文斯蓝法测定伤后4h肾组织通透性及含水量的变化,检测血肌酐(Cr)及血细胞压积(HCT)变化。结果烫伤后4h单纯烫伤组,氯化钠组肾伊文斯兰含量、Cr、HCT及含水量较假烫组明显增加(P<0.05),丙酮酸钠组伊文斯兰含量、Cr、HCT及含水量较单纯烫伤组、氯化钠组明显降低(P<0.05),而与假烫组无明显统计学差异。结论丙酮酸钠能明显减轻烧伤早期血管通透性的增加,有助于烧伤后休克及并发症的防治。

【Objective】 To observe the effect of pyruvate on the kidney vascular permealbility in rats with scalded injury.【Methods】 SD rats were randomLy divided into foue group ：sham control,scald alone,scald plus fluid resuscitation and scald plus fluid resuscitation pyruvate group.There are 6 rats at each group.A rat model of 50% was established.The survival rats were examined for pulmonary vascular permeability with Evans Blue straining method.Specimens of kidney were harvested for determination of water content with oven drying method.Bloods were collected for tests of HCT and Cr.【Results】 kidney vascular permeability,water content,HCT and Cr at the time of 4 hours after scald in scald alone and scald plus fluid resuscitation were significantly increased（P0.05）,and those in pyruvate group were significantly decreased when compared with those in scald alone and scald plus fluid resuscitation（P0.05）,while no significantly difference was found compared sham control group.【Conclusion】 pyruvate can significantly inhibit the increases of the kidney vascular permealbility in rats with scalded injury,and it would be helpful for the prevention of shock after scald and complication.