电子自旋共振和脂质过氧化对亚硒酸钠诱发大鼠白内障的自由基作用研究

Free Radicals on Cataracts Induced by Sodium Selenite Studied by ESR and LPO

以 Ｎａ２ Ｓｅ Ｏ３ 诱发大鼠白内障，用 Ｅ Ｓ Ｒ 波谱仪测到了自由基信号（ｇ＝ ２．００２６），发现注射 Ｎａ２ Ｓｅ Ｏ３后晶体自由基浓度显著高于正常水平，而白内障后期低于晶体正常水平。结果提示，在晶体氧化损伤初期给予抗氧化剂，对白内障的形成有预防和对抗作用，但是在白内障晚期则可能会促进白内障的发展。

We made a electron spin resonance (ESR) study on rat cataracts induced by Na 2SeO 3. A signal was detected at about g=2.0026, and the content of electron spins (CES) in lens of rat administrated Na 2SeO 3 for a short time is higher than that of control significantly. At the late stage of cataracts, the content of CES was lower than normal level. The results of kinetics analysis showed that the CES grow up with the administration of Na 2SeO 3 and decays in index law after administration. The results suggested that free radical scavengers or anti oxidants should be useful for preventing cataracts at initial stage of the oxidation damage to lens, but they could aggravate cataracts at late stage of cataracts. This can explain the controversy about the curative effect of anti oxidant to cataracts.