静脉丙种球蛋白对小鼠病毒性心肌炎T细胞亚群、NK细胞及部分细胞因子的影响研究

Influence of Intravenous Immunoglobulin on T Lymphocyte Subsets, NK Cell and Some Cytokines in Mice with Experimental Viral Myocarditis

为探讨病毒性心肌炎（ Ｖ Ｍ Ｃ）免疫发病机理及静脉丙种球蛋白（ Ｉ Ｖ Ｉ Ｇ）治疗 Ｖ Ｍ Ｃ的作用机理，将 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠随机分为正常对照组、病毒模型组 、 Ｉ Ｖ Ｉ Ｇ 保护组及 Ｉ Ｖ Ｉ Ｇ 治疗组，观察 Ｉ Ｖ Ｉ Ｇ 对小鼠脾脏 Ｔ 细胞亚群、 Ｎ Ｋ 细胞、 Ｉ Ｌ１、 Ｉ Ｌ２及外周血 Ｔ Ｎ Ｆ活性的影响。结果：与正常对照组比较，病毒模型组 Ｎ Ｋ Ｃ活性及 Ｃ Ｄ３、 Ｃ Ｄ４ 、 Ｃ Ｄ８均明显降低， Ｉ Ｌ１、 Ｉ Ｌ２及 Ｔ Ｎ Ｆ活性明显升高，而 Ｉ Ｖ Ｉ Ｉ Ｇ 保护组及治疗组的上述指标均有明显改善。结论： Ｔ 细胞介导的细胞免疫反应在病毒性心肌炎发病机理中起着重要作用。 Ｉ Ｖ Ｉ Ｇ 能有效地改善并调节病毒性心肌炎的免疫功能，为 Ｖ Ｍ Ｃ安全有效的治疗手段。

This study sought to gain an insight into the immunological pathogenesis of viral myocarditis and the mechanism of therapeutic action of intravenous immunoglobulin (IVIG) on the disease, BALB/c mice were randomized into four groups: normal control group; “myocarditis model group”, incoculated intraperitoneally with CVB3; “IVIG protection group”, injected intraperitoneally with CVB3 and IVIG; and “IVIG treatment groups”, injected intraperitoneally with CVB3 and IVIG. The amount of T cell in different T cell subsets and the activities of NK cell, IL 1and IL 2 were assayed using spleen specimen while peripheral blood was used to measure TNF activity. The results showed:that in comparision with normal control, all spleen T cell subsets decreased in amount in myocarditis model group, so did its NK cell activity while its IL 1, IL 2 and TNF activities increased significantly.When compared with myocarditis model group, however, both IVIG protection group and IVIG treatment group showed significant elevation of NK cell activity and T cell subsets but reduced IL 1, IL 2 and TNF activities with no remarkable change in T cell subsets. The results of suggest that the immunological injury mediated by T cells plays a critical role in the pathogenesis of viral myocarditis. The protective and therapeutic effects of IVIG on the murine CVB3 myocarditis and the related immunological evidence of its actions may indicate the prospect that IVIG will become a potent and safe treatment for viral myocarditis in human.