不同配方胃肠外营养对大鼠肝部分切除术后肝再生的影响

Influence of Different Total Parenteral Nutrition Support upon Hepatic Regeneration after Partial Hepatectomy in Rats

为探讨不同配方胃肠外营养支持对肝部分切除术后肝再生的影响及其机理，将大鼠随机分为４组， Ａ 组正常切肝后用强化支链氨基酸胃肠外营养（强化 Ｂ Ｃ Ａ Ａ Ｔ Ｐ Ｎ）治疗； Ｂ组正常肝切除后用标准胃肠外营养（标准 Ｔ Ｐ Ｎ）治疗； Ｃ、 Ｄ组制成肝硬化模型后切肝和营养支持分别同 Ａ、 Ｂ组；术后第三天处死动物测定肝再生情况。结果：肝 Ｄ Ｎ Ａ 合成率和有丝分裂率在 Ａ、 Ｂ组分别显著高于 Ｃ、 Ｄ组；肝再生率、 Ｄ Ｎ Ａ 合成率及有丝分裂率在 Ａ、 Ｃ组分别显著高于 Ｂ、 Ｄ组。以上提示：切肝后在相同胃肠外营养支持下硬化肝再生较正常肝差；在强化 Ｂ Ｃ Ａ Ａ Ｔ Ｐ Ｎ 支持下正常肝和硬化肝的再生均优于标准 Ｔ Ｐ Ｎ

This study was designed to investigate the effects of different TPN on liver regeneration after partial hepatectomy in rats. Forty Wistar rats were randomyly divided intio four groups. Groups A and B (normal rats) received BCAA enriched TPN (45% BCAAs) and standard TPN (24% BCAAs) respectively after 34% hepatectomy; Groups C and D (rats with cirrhosis of liver) received the same TPN as Groups A and B received respectively after 34% hepatectomy. On the third day after operation, all rats were killed and specimens of liver and blood were taken for measurement of activity of liver regeneration. The results showed that DNA synthesis rate and mitosis rate in Groups A and B were significantly greater than those in Groups C and D respectively; RLR, DNA synthesis rate and mitosis rate in Groups A and C were significantly higher than those in Groups B and D respectively. These suggest that the effect of the same TPN on liver regeneration in normal rats is better than that in cirrhosis rats and that BCAA enriched TPN can provide more excellent supplementation than standard TPN can do either in normal liver or cirrhosis liver after partial hepatectomy.