大黄对烧伤模型小鼠疗效及其机理的探讨

Studies of the Effect and the Mechanism of Sennosides in the Burn Mice Model

本文旨在研究大黄对烧伤的疗效及其作用机理。 2 6只Balb/c小鼠 ,制成烧伤模型 ,随机分成大黄汤剂治疗组和对照组。专人盲法测定肠道灌洗液中总蛋白和球蛋白 (G) ,同时送小肠病理学检查。结果发现 :①治疗组小鼠存活率较对照组有所提高 ;②病理显示对照组 ,小肠绒毛变平 ,有破损现象 ,而用了大黄的小鼠肠粘膜也有破损 ,但可见更多的完整的肠绒毛存在 ,且肠绒毛较对照组高 ;③治疗组小鼠肠道灌洗液中 ,总蛋白和球蛋白的含量均明显高于对照组 ,其均数分别为 3 72g/L和 1 5 7g/L (P <0 0 5 )及 4 12g/L和 2 13g/L (P <0 0 5 )。提示严重烧伤可导致肠粘膜屏障损害。而大黄有促肠道球蛋白分泌作用 ,及减轻肠道形态学改变的作用 。

Object:To investigate the effect and the mechanism of sennosides in the burn mice model.Methods:26 Balb/c mices were made into the model of burn mice and were classed into treatment group with decoction of sennosides and control group.The total protein and globulin in the saline which was gavaged though the whole small intestine were measured,while the tissue histologic studies of small intestine was perfurmed.Results:①The survival rate in treatment group is higher than that in control group.②The tissue histologic findings showed shortening,fragmentation and atrophy of villi in control group.Some fragmentation and degeneration were observed but the small intestine showed more intact villi and more uniform villus structure and villi were longer in treatment group.③The average number of globulin in test group and in cantrol group were 3 72g/L and 1 57g/L,respectively.There was statistical difference( P <0 05)between the groups.The average number of total protein in test group and in control group were 4 12g/L and 2 13g/L,respectively.There was statistical difference( P <0 05)between the groups.Conclusions:Severe burn injury may damage the barrier of the intestinal mucosa.Sennosides may improve the secretion of mucus and globulin of the intestine and partially prevented the morphologic alteration in the intestinal mucosa 24 hours after burn injury,which decreased the mortality rate of burn injury.