柯萨奇B组病毒感染与小儿哮喘发作关系的探讨

A study of the relationship between Coxsackievirus group B infection and asthma attack in children

目的 探讨柯萨奇 Ｂ 组病毒（ Ｃ Ｖ Ｂ） 感染与哮喘发作的关系。方法 观察急性发作期哮喘患儿１０２ 例、上呼吸道感染１８２ 例、非感染性疾病患儿７１ 例、健康儿童３０ 例。采用 Ｅ Ｌ Ｉ Ｓ Ａ 法测定静脉血 Ｃ Ｖ Ｂ 抗原（ Ｃ Ｖ Ｂ－ Ａｇ）和 Ｉｇ Ｍ 抗体（ Ｃ Ｖ Ｂ－ Ｉｇ Ｍ） 、免疫组化法测定 Ｔ 细胞亚群和［３ Ｈ］ － Ｔｄ Ｒ 标记法测定 Ｎ Ｋ 细胞活性。结果 ①哮喘患儿 Ｃ Ｖ Ｂ 感染率为５７８ ％ （５９／１０２） ；②哮喘患儿 Ｃ Ｄ８ 显著升高； Ｃ Ｄ３ 、 Ｃ Ｄ４／ Ｃ Ｄ８ 及 Ｎ Ｋ 细胞活性均明显下降（ Ｐ ＜００１） ；而 Ｃ Ｖ Ｂ 阳性哮喘患儿 Ｎ Ｋ 细胞活性又低于非 Ｃ Ｖ Ｂ 阳性哮喘儿（ Ｐ ＜ ００５） ， Ｃ Ｄ８ 明显高于非 Ｃ Ｖ Ｂ 阳性哮喘儿（ Ｐ ＜ ００１） 。结论 Ｃ Ｖ Ｂ 感染与哮喘发作有密切关系。

Objective To study the relationship between Coxsackievirus group B infection and asthma attack in children .Methods 102 cases of asthma attack,182 cases of upper respiratory tract infection,71 cases of other kinds of diseases and healthy children (30 cases) were detected antigen (CVB-Ag) and IgM (CVB-IgM) using ELISA method,T cell lymphatic subpopulations using immune chemical method,and NK cell activity using [ 3H]-TdR method.Results 1.The positive incidence of CVB was 57 8%(59/102),which was higher than that of other three groups (42 3% of upper respiratory tract infection,P<0 05;17% of other kinds of diseases,P<0 01;3% of healthy children,P<0 01).2.There were lower NK cell activity (P<0 01) and confusing of T cell lymphatic subpopulations in the children with CVB positive in asthma attack (P<0 01).Conclusion There is a close relationship between Group B Coxiekieviruses infection and asthma attack in children.