变温对鱼类抗氧化防御影响的Meta分析

A meta-analysis of the effects of temperature variation on antioxidant defenses in fish

检索中国期刊全文数据库(1994.1～2009.8)、万方数据库(1980.1～2009.8)、维普数据库(1989.1～2009.8),以及Scopus(1960.1～2009.8)、Elsevier(52009.8)、SpringerLink(52009.8)和Blackwell(52009.8)数据库,系统收集涉及温度变化导致鱼类组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、谷胱甘肽还原酶(GR)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和丙二醛(MDA)变化的相关研究,对纳入23篇文献进行了数据提取和Meta分析,并系统评价变温对鱼类抗氧化防御的影响。除GSSG由于研究数太少不能分析外,Meta分析显示:升温显著提高SOD(标准化均数差SMD=1.0,95%CI=0.4～1.7,P=0.001)和GPX(SMD=0.4,95%CI=0.1～0.7,P=0.005)活力,降温显著下调GPX(SMD=-0.9,95%CI=-1.7～.0.1,P=0.025)和GR(SMD=-1.6,95%CI=-2.5～.0.8,P<0.001)活力。升降温对CAT活力和GSH均无显著影响(P>0.05),但都会显著增加MDA水平(SMD=I.2～1.4,P<0.006)。不同鱼类、组织和测定方法不是引起研究异质性的主要因素,但试验设计的变温幅度是产生SOD、CAT和MDA研究间异质性的主要因子,实验开始温度也会引起GSH研究间的异质性。

Studies on superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GPX）, glutathione reductase （GR）, reduced glutathione （GSH）, oxidized glutathione （GSSG） and malonaldehyde （MDA） were searched from the Chinese Journal Full-text Database （1994.1 - 2009.8）, WanFang Database （1980.1 ~ 2009.8）, WeiPu Database （1989.1 - 2009.8）, as well as Scopus （1960.1 - 2009.8）, Elsevier （-2009.8）, SpringerLink （~2009.8） and Blackwell （-2009.8） Database. Data from a total of 23 studies indexed with antioxidant and poecilothermia were systematically collected. Those extracted by two reviewers independently were subject to a meta-analysis, and a systematic review on antioxidant defenses in fish was finally conducted with Stata 8.0 software. GSSG was excluded due to the low number of study cases. The meta-analysis showed that SOD [SMD （standardized mean difference）=1.0, 95% CI--0.4～ 1.7, P=-0.001] and GPX （SMD=0.4, 95% CI=0.1 - 0.7, P=0.005） activities increased significantly with increasing temperature, while GPX （SMD= -0.9, 95% CI= -1.7 - -0.1, P=0.025） and GR （SMD= -1.6, 95% CI= -2.5～ -0.8, P〈0.001） were significantly reduced when temperature dropped. Either an increase or decrease in temperature had no obvious difference in CAT activities and GSH levels （P〉0.05）, but caused a marked increase in MDA levels （SMD=I.2 - 1.4, P〈0.006）. Across all study cases, the heterogeneity of the results was unrelated to fish species, tissues, and assay methods. However, a meta-regression analysis indicated that the magnitude of experimental temperature variation accounted for the heterogeneity among studies related to the SOD, CAT activities and MDA levels. Moreover, initial temperatures in trials were another factor responsible for the heterogeneity of the GSH results.