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梅花鹿TGF-β1基因的克隆及序列分析

Cloning and Sequencing Analysis of TGF-β1 Gene in Sika Deer
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摘要 从梅花鹿的鹿茸血中提取总DNA,经PCR扩增后,对梅花鹿的TGF-β1基因部分片段进行了克隆测序,序列长度为839 bp,现已将该序列提交到Gene Bank上。Gene Bank中的Blast分析表明,梅花鹿TGF-β1基因与牛同源性为92%,与羊同源性为94%。 The paper studies to separate and extract the overall DNA from pilose antler blood,and part of segments of TGF-β1 Gene in Sika Deer were cloned and sequenced after PCR amplification with length of 839 bp,and now the sequence has been sent to GenBank with registration.Comparison the fragment sequence with that of cattle and sheep TGF-β1 gene was carried out through blast of GenBank,the result showed that the homolgies of the nucleotide sequence was 92% with cattle and 94% with sheep.
作者 陈斌 徐细建 李馨
机构地区 黑龙江八一农垦大学动物科技学院
出处 《黑龙江八一农垦大学学报》 2011年第3期40-42,共3页 journal of heilongjiang bayi agricultural university
关键词 梅花鹿 TGF-Β1基因 克隆测序 sika deer TGF-β1 gene cloning and sequencing
分类号 S825 [农业科学—畜牧学]
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参考文献11

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黑龙江八一农垦大学学报
2011年 第3期

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