同时提取土壤微生物DNA和RNA方法的研究

Simultaneous Recovery of DNA and RNA from Soil Microbe

获得高浓度、大片段、多样性程度高的土壤微生物总DNA是研究土壤微生物群落结构的分子生态学基础。研究采用PBS缓冲液洗涤土壤样品,结合SDS裂解微生物细胞的方法,同时提取分别添加小麦秸秆粉和油菜秸秆粉的两种土壤样品的微生物DNA和RNA。对提取出的DNA和RNA进行酶切,基因组PCR和反转录PCR,实验结果表明该法提取的核酸不需要进一步处理,其纯度可以满足后续的分子生物学试验的研究,同时避免了由于纯化导致的核酸量的降低。为研究土壤微生物多样性和土壤微生物的活性及在土壤环境中的功能等方面的研究奠定了基础。

Isolation of DNA from high purity,large fragment and diversity of soil microbe is the basis for molecular ecology study on community structure.PBS washing procedure and SDS extraction method were chosen to extract DNA and RNA from two types of soils simultaneously.Enzyme digesting,PCR and RT-PCR for the extracted DNA or RNA,and results showed that purity of nucleic acid was suitable for follow-up procedures,which would provide basis for studying of microbial activities and function in soil environment.