摘要
目的 用套式 P C R 快速检测水沟和丛林等地的钩端螺旋体。方法 选 Leptospira borgpetersenii 中的一段保守序列 I S1533 , 设计两对引物, 用套式 P C R 检测六种不同血清型的钩体。结果 6 株致病株均出现单一246bp 的特异性扩增产物, 而腐生株、空白对照无任何 D N A 扩增条带。用琼脂糖凝胶电泳, 能检测500fg 模板的扩增产物。结论 套式 P C R 是一种灵敏度高、特异性强的快速检测自然疫源中钩体的有效方法。
Aim To rapidly detect Leptospira in ditch and jungle by nested PCR.Methods Two sets of primers were derived from an inserted sequence IS1533 of Leptospira borgpetersenii.Six serotypes Leptospires were detected by nested PCR.Results\ A specific amplification product with 246bp length could be found from six serovar pathogenic Leptospires by nested PCR,and agarose gel electrophoresis is demonstrated to have a sensitivity of detecting 500fg DNA amplification products.Conclusion\ Nested PCR is a rapid,specific and sensitive method for detecting Leptospira in the epidemic regions.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第5期18-20,共3页
Chinese Journal of Zoonoses
基金
全军医药卫生科研基金