恙螨体内HFRSV基因检测及定位的初步研究

STUDY ON DETECTION,PROLIFERATION AND LOCATION OF HFRSV IN CHIGGER MITE

１９９７ 年以来我们采用分子生物学方法对小盾纤恙螨的媒介意义进行研究。结果表明， 用 Ｒ Ｔ－ Ｐ Ｃ Ｒ 和 Ｎｅｓｔｅｄ Ｒ Ｔ－ Ｐ Ｃ Ｒ 从恙螨体内检测到 Ｈ Ｆ Ｒ Ｓｒ－ Ｒ Ｎ Ａ； 用原位分子杂交技术在恙螨的卵巢细胞、支肠细胞等组织中检测到 Ｈ Ｆ Ｒ Ｓ Ｕ－ Ｒ Ｎ Ａ。以上结果为恙螨的媒介意义提供了具有分子水平的直接证据， 对 Ｈ Ｆ Ｒ Ｓ 的流行病学和预防具有重要的理论意义和实用价值。

Chigger mite had been studied as a vector by molecular biological methods since 1994.The results show that HFRSV-RNA could be detected by RT-PCR and Nested RT-PCR in chigger mite.TCID 50 /ml titer of HFRSV in larva and nymph detection demonstrated that HFRSV could be transmitted by trans-stadial and proliferated in chigger mites.HFRSV-RNA located in ovary cells and midgut cells of chigger mites.The above results indicated that chigger mite is a transmitting vector of HFRSV.