用Vero-E6细胞大量增殖恙虫病立克次体的研究

STUDY ON LARGELY REPRODUCING ORIENTIA TSUTSUGAMUSHI WITH VERO-E6 CELL

目的 为了探讨建立一种简便而可靠的恙虫病立克次体增殖方法， 以得到较大量的恙虫病立克次体。方法 采用了以 Ｖｅｒｏ － Ｅ６ 细胞增殖恙虫病立克次， 并且对 Ｖｅｒｏ － Ｅ６ 感染细胞的培养条件进行了改进。结果 成功地使恙虫病立克次体在 Ｖｅｒｏ － Ｅ６ 细胞中得到大量增殖。结论 改进后的 Ｖｅｒｏ － Ｅ６ 细胞培养法可以获得大量的恙虫病立克次体， 该方法既经济又适用。

AimTry to find a simple and reliable method of reproducing Ot to obtain large amount of Ot.MethodVero-E6 cell was used to reproduce Ot,and the cultural condition for infected Vero-E6 cells has been improved.Result Ot was largely reproduced successfully in Vero-E6 cells .ConclusionThe improved Vero-E6 cells cultured method can produce large am ount of Ot.The method is economic and practical as well.