宫颈癌患者人乳头瘤病毒感染分布特点及临床研究

Study on the Distribution and Clinical Research of Human Papillomavirus Infection in Women Patients with Cervical Cancer

目的:探讨人乳头瘤病毒(HPV)各亚型在广西沿海地区宫颈癌患者中的分布情况,HPV感染与宫颈癌患者的年龄、临床分期、病理类型、分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发的关系,为广西沿海地区妇女宫颈癌的防治提供理论依据。方法:通过凯普导流杂交HPV DNA检测法,对76例宫颈癌患者宫颈脱落细胞进行21种HPV亚型的检测。结果:宫颈癌HPV总阳性率为90.8%。宫颈癌患者HPV阳性各亚型出现的频率排序为:HPV16(56.5%),HPV18、33、58各(7.2%),HPV52、53各(5.8%),HPV31(4.3%),HPV45(2.9%),HPV35、51、56、66、68各(1.4%)。HPV6(5.8%),HPV11、44、43各(1.4%)均合并在高危感染中。HPV感染与临床分期、肿瘤分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发差异无显著性(P>0.05),与年龄密切相关,鳞癌HPV阳性率明显高于腺癌及其它癌,差异有统计学意义(P<0.05)。结论:广西沿海地区妇女宫颈癌患者中以HPV16、18、33、58感染为主要型别。HPV感染与宫颈癌的临床分期、肿瘤分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发无明显相关性,与发病年龄、病理类型有关。

Objective：To discuss the distribution of human papillomavirus （HPV） and each subtype among women patients with cervical cancer in Guangxi coastal areas,the relations between the ages, clinical stages, pathological types,differentiation degrees,among patients with HPV infection and cervical cancer, so as to provide theory basis for clinical prevention and treatment of cervical cancer in costal areas.Methods：Adopting flow-through hybridization HPV DNA detection method to undertake 21 HPV subtypes diagnosis with cervix cell from 76 patients with cervical cancer.Results：The total positive HPV rate was 90.8%. The occurring frequency of each subtype of positive HPV was separately HPV16（56.5%）,HPVI8,33,58 （7.2）,HPV 52,53（5.8%）,HPV 31（4.3%）,HPV 45 （2.9%）,HPV 35,51,56,66,68（1.4%）.HPV6 （5.8%）,HPVI 1,44,43（1.4%） were all combined in the high risk of infection.The difference between HPV infection and clinical stages,tumor differentiation, tumor pelvic lymph node metastasis and tumor relapse had no significance（P〉0.05）,but had close relations with the age.The positive HPV rate of squamous cell carcinoma was distinctly higher than that of adenocarcinoma and other carcinoma,and the difference was of statistics significance（P〈0.05）.Conelusion：In Guangxi coastal areas,women patients with cervical cancer suffer the major types of HPVI6,18,33 and 58.There was no relationship between HPV infection and clinical stages, tumor differentiation,tumor pelvic lymph n6de metastasis and tumor relapse,but involved in age and pathological types.