柴油污染地下水修复生物反应墙中功能微生物数量及群落多样性

Quantity and Community Diversity of Functional Microbes in Biobarrier Remediating of Diesel Contaminated Groundwater

利用功能微生物、泥炭和含水层介质构建生物反应墙原位修复柴油污染地下水,系统经调试稳定后运行80 d,目标污染物去除率为83.60%~99.85%。运行期结束,采用荧光素-最大或然数和Biolog方法研究了生物墙内不同位置处功能微生物的数量及群落多样性。结果表明：墙体内各处均能保持较高的功能微生物数量,且微生物群落的物种数、丰富度、均一性、碳源利用性以及代谢特征均相似;但随着生物墙深度的增加,功能微生物比例有所下降,微生物群落略显丰富,但均一性略差,碳源利用倾向略微不同。生物反应墙原位修复地下水时,功能微生物能够长期保持较高的数量、较好的降解性能和稳定的群落多样性;但随着墙体深度的增加,营养结构发生了改变,导致微生物群落结构略显不同。

Functional microbes,peat,and aquifer medium were used to construct biobarrier for in-situ remediation of diesel contaminated groundwater.The system removal efficiency of target contaminants could reach 83.60%-99.85% during the 80 day steady operation.FDA-MPN and Biolog methods were adopted to study the quantity and community diversity of the functional microbes at different positions in biobarrier after the complete operation.The results indicated that each part of biobarrier had abundant functional microbes,and the microbial community in the structures had a resemblance in species,richness,homogeneity,carbon source utilization,and catabolic characteristics.However,the structure of microbial community decreased a little as biobarrier became deeper,as the proportion of functional microbes decreased,the microbial community became richer,the homogeneity got worse,and utilization tendency of carbon showed differently.The functional microbes in biobarrier could maintain relatively higher microbial quantity,degradatable properties and stable community diversity for a long time.Whereas,with nutrients structure changed deeper in biobarrier,community diversity structure of functional microbes showed a little differently when system in-situ remediate diesel contaminated groundwater.