肺表面活性物质相关蛋白A与D在肺结核诊断中的研究

A study of pulmonary surfactant associated protein A、D for diagnosis in pulmonary tuberculosis

目的研究肺表面活性物质相关蛋白A、D(pulmonary surfactant associated protein A、D,SP-A、SP-D)在肺结核患者中的表达,探讨2者的相关性。方法采用ELISA方法检测涂阳肺结核50例,涂阴肺结核51例,慢性阻塞性肺疾病(COPD)患者30例及健康人85名血清及痰液中SP-A、SP-D的表达情况。结果涂阳肺结核组痰液中SP-A、SP-D的表达与痰液中结核分枝杆菌的含菌量有相关性;血清SP-A、SP-D在肺结核组高表达(P<0.05),痰SP-D在肺结核组低表达(P<0.01)。结论 SP-A、SP-D的表达与肺结核之间可能有相关性,或可成为协助肺结核诊断的辅助指标。

Objective To study the expression of pulmonary surfactant associated protein A、D（SP-A、SP-D） and their correlation in patients with pulmonary tuberculosis Methods The expression of SP-A、SP-D of sputum and serum in 50 patients with smear positive pulmonary tuberculosis,51 patients with smear negtive pulmonary tuberculosis,30 patients with COPD and 85 healthy volunteers were detected by ELISA. Results The expression of SP-A、SP-D of sputum in patients with smear positive pulmonary tuberculosis were correlated with the amount of mycobacterium tuberculosis in sputum from these patients.The expression of SP-A、SP-D of serum in patients with pulmonary tuberculosis were significant higher than that of others（P0.05）.But the expression of SP-D of sputum in patients with pulmonary tuberculosis were significant lower than that of others（P0.01）. Conclusions The expression of SP-A、SP-D in patients are correlated with pulmonary tuberculosis.They may be an adjuvant marker to diagnose pulmonary tuberculosis.