钆标记抗人肺癌单抗的磁共振免疫成像研究

MR Immunoimaging Using Antihuman Lung Cancer Monoclonal Antibodies Labelled Gadolinium

目的：探讨Ｇｄ标记抗肺癌单抗对荷瘤裸鼠的定向增强效应。材料与方法：用ＤＴＰＡ环二酸杆与抗肺癌单抗３Ｄ３和Ｇｄ＾３＋分别偶联，制备出Ｇｄ－ＤＴＰＡ－ＩｇＧ；建立６只荷瘤裸鼠模型；将Ｇｄ－ＤＴＰＡ－ＩｇＧ按２ｍｇ单抗／只的剂量从尾部脉内淳入；观察平扫扩注药后６小时瘤体磁共振信号。结果：Ｇｄ－ＤＴＰＡ－ＩｇＧ能选择性增加瘤灶的信号强度。结论：磁共振免疫成像为肺癌定性提供一种潜在新的方法。

Purpose: To explore the Possibilitv of specific contrast enhancemen1 in nude mice hosted lung cancer using Gd labelled monocloa1 antibodies against lung adenocarcinorna. Materials and Methods: Uslng cyclic anhydride DTPA as a bifunctional chelat1ng a- gent, Gd3+ was attached to a monoclnal antihody (3D3). 6 nude mlce carrying the 1ung cancer were esta[)lished. Then, Gd - DTPA - IgG was intravenously injected at a dose of 2mg IgG/mouse. MR scans wcre performed before and 6 hours after administration of Gd - DTPA - IgG. ResultS: all tumor of nodules were specifically cnhancecl with Gd - DTPA - IgG. ConcIusion: MR immunoimaging is a promise method for detecting lung cancer.