大鼠同源异型盒基因hex的克隆与序列分析

Cloning and Sequence Analysis of Homeobox Hex Gene in Wistar Rat

Hex基因作为同源异型盒基因超家族的成员,在造血细胞的分化和发育中发挥着重要的作用。为研究该基因在乳腺发育和分化中的作用,本研究采用RT-PCR方法扩增大鼠hex基因,并克隆到pMD18-T载体后测序。结果表明,大鼠的Hex基因读码框由816 bp组成,编码271 aa,分子质量为30 ku。与已发表的大鼠该基因序列完全一致,与小鼠、禽类、人、非洲爪蟾和斑马鱼相比氨基酸序列的同源性在70.7%-97.0%之间。进化树显示,大鼠与小鼠的Hex基因在同一分支内,表明该蛋白保守,在物种进化中具有重要的作用。

Hex（haematopoietically expressed homeobox,hex）gene has been found to carry out cellular differentiation and development in haematopoietic system.To study the function of hex gene in mammary gland development,hex gene from Wistar rat was amplificated by RT-PCR and cloned into pMD18-T in the study.The result indicated that the open reading frame of rat hex gene was 816 bp in length which could encode 271 amino acids,and the molecular weight of HEX protein was 30 ku.It was same as the sequences of hex gene published in GenBank.The homology of amino acids were 70.7%-97.0% with chicken,human,mouse,xenopus and zebrafish.The phylogenetic tree of HEX protein indicated that rat and mouse were in the same sub-group and proved that HEX protein was conserved in sequence and had important function in evolution.