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奶山羊IL-1β在Sf9细胞中的表达

Expression of Diary Goat IL-1β in Sf9 Cells
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摘要 将CaIL-1β基因整个编码区cDNA亚克隆到杆状病毒载体pFastBac-HTb中,构建转移载体pFastBac-HTb-CaIL-1β,转座到宿主菌DH10 Bac中,在Kan+/Gm+/Tet+/X-gal/IPTG LB平板上筛选白色菌落,碱裂解法提取质粒即为穿梭载体Bacmid-CaIL-1β,转染Sf9细胞后获得有感染力的重组病毒re-Bac-CaIL-1β。重组病毒在Sf9细胞上来连续传代后,经Western blot检测,CaIL-1β主要表达于感染的Sf9细胞培养上清中。 The fragment of CaIL-1β was subcloned into baculovirus vector pFastBac-HTb,recombinant transfer vector pFastBac-HTb-CaIL-1β was transformed into DH10 Bac,by site-specifc transposition.CaIL-1β gene was integrated into Bacmid,the recombinant shuttle vector Bacmid-CaIL-1β was got.The reBac-CaIL-1β was got by transfecting Sf9 cells with shuttle vector.Western blot assay suggested that the recombinant CaIL-1β exsit in the supernatant of reBac-CaIL-1β infected Sf9 cells.
作者 姚婧 赵莉娟 敬晓棋 赵光辉 陈德坤
机构地区 西北农林科技大学动物医学院 榆林学院生命科学学院
出处 《动物医学进展》 CSCD 北大核心 2011年第6期44-47,共4页 Progress In Veterinary Medicine
基金 公益性行业(农业)专项科研经费项目(201103038)
关键词 奶山羊 IL-1Β SF9细胞 diary goat IL-1β Sf9 cells
分类号 S852.4 [农业科学—基础兽医学] S858.27 [农业科学—临床兽医学]
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参考文献15

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二级参考文献23

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动物医学进展
2011年 第6期

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