鸡白痢沙门菌和鸡伤寒沙门菌PCR-RFLP鉴别

Differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP

根据鸡白痢沙门菌和鸡伤寒沙门菌flic基因可变区两端的保守序列设计1对引物,PCR扩增出约866 bp的产物,用Hinp1I对PCR产物进行酶切,经RFLP分析区分鸡白痢沙门菌和鸡伤寒沙门菌。利用该技术对1株鸡白痢沙门菌标准株及2株鸡伤寒沙门菌标准株进行分子鉴别,结果与预计的RFLP模式相符,证明该方法可行。在此基础上对分离株进行鉴别,证明分离株均属于鸡白痢沙门菌。

According to the conserved sequence of the variable region of flic gene from S.pullorum and S.gallinarum,a pair of specific primers were designed.The PCR amplified product,866 bp fragment was digested with Hinp1I.S.pullorum and S.gallinarum were able to be differentiaed by PCR-RFLP.By using this method,the reference strains of S.pullorum and 2 reference strains of S.gallinarum were identified,with the results matching the predicted patterns.It could be concluded that this method is feasible.Furthermore,the isolates were differentiated,all of them were S.pullorum.