麝香保心丸对兔动脉壁一氧化氮代谢的影响

Effect of heart-protecting musk pill on nitric oxide metabolism of arterial wall in cholesteriol-fed rabbits

目的 为阐明麝香保心丸改善心肌血流灌注的机理。方法 在高脂血症造成兔动脉壁一氧化氮代谢异常的动物模型上，采用定量ＲＴ－ ＰＣＲ 等技术，观察了该药的干预作用。结果 高脂饮食可造成动脉一氧化氮代谢的紊乱，麝香保心丸可部分逆转这种损害；与高脂血症对照组相比，它有提高动脉壁内皮型一氧化氮合酶（ｅＮＯＳ） 基因表达的趋势（０－１＞ Ｐ＞ ０－０５） 、显著增强动脉的ＮＯＳ 的活力（ Ｐ＜ ０－０５） 、提高血浆一氧化氮代谢产物（ＮＯＰ） 的水平（Ｐ＜０－０５） 。结论 麝香保心丸的抗心肌缺血作用可能与保护动脉壁一氧化氮系统有关。

Objective In order to examine the mechanism of heart-protectin musk pill (HMP) in increasing blood perfusion of the ischemic myocardium .Methods We studied the effect of HMP on nitric oxide (NO) metabolism of arterial wall in cholesteriol-fed rabbits .The level of endothelial nitric oxide synthase (eNOS) mRNA was detected by quantitative reverse transcription polymerase chain reaction(RT-PCR). Nitric oxide synthase (NOS)activity of arterial wall and the concentration of plasma nitric oxide production (NOP) were measured in three groups (Control group (C)n=8,Hypercholesterolemia group(HC)n=8 and HMP group (SX)n=9).Results Indicate that hyperlipidemia caused decrease in eNOS mRNA levels and NOS activity (compared with C group, P< 0 05) It reduced the quantity of NOP simutanuoesly (compared with C group , P< 0 05) HMP can partly reverse the impairement of NO system .It had a tendency of increasing eNOS mRNA levels (compared with HC group, 0.1> P> 0 05)and markedly elevated the NOS activity and the concentration of NOP (compared with HC group, P< 0.05).Conclusion This observation supports the hypothesis that the effect of increasing myocardium perfusion of HMP may be related to improve NO metabolism. [