食管鳞癌组织中RhoC mRNA和蛋白的表达及意义

Expression and significanse of RhoC and protein in esophageal squamous cell carcinoma

目的探讨食管鳞癌、癌旁不典型增生及正常食管黏膜组织中RhoC mRNA和蛋白的表达及其与临床病理因素的关系。方法应用原位杂交、免疫组化法检测62例食管鳞癌、31例癌旁不典型增生及62例正常食管黏膜组织中RhoC mRNA和蛋白的表达情况。结果在癌组织、癌旁不典型增生及正常黏膜组织中RhoC mRNA和蛋白的表达率依次降低,组间比较差异有统计学意义(P<0.05),其高表达与食管鳞癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05);RhoC mRNA与蛋白的表达呈正相关关系(P<0.05)。结论 RhoC mRNA和蛋白的高表达可能在食管鳞癌的发生发展过程中起重要作用,可望作为判断食管鳞癌侵袭转移能力的一项客观指标。

Objective To investigate the clinicopathological of RhoC mRNA and protein expression in esophageal squamous cell carcinoma（ESCC）,cancer-adjacent atypical hyperplasia epithelium and normal esophageal epithelium.Methods The expression of RhoC mRNA and protein in 62 cases of esophageal squamous carcinoma,31 cases of cancer-adjacent atypical hyperplasia epithelium and 62 cases of normal esophageal epithelium tissues were detected with in situ hybridization and SP immunohistochemical method.Results The expression of RhoC mRNA and protein decreased in turns in carcinoma,adjacent atypical hyperplasia epithelium and normal esophageal epithelium specimens,there were significant differences in group comparison（P0.05）.For ESCC,the expression level of RhoC mRNA and protein were significantly related to the degree of differentiation,depth of invasion and lymph node metastasis（P0.05）.There was the significant positive correlation between RhoC mRNA and RhoC protein（P0.05）.Conclusion The increased expression of RhoC mRNA and protein may play an important role in carcinogenesis,invasion,metastasis of esophageal squamous cell carcinoma.RhoC may become an objective target to evaluate the potential of lymph node metastasis of esophageal squamous cell carcinoma.