红掌茎段侧芽离体快繁技术研究

Technique for in vitro Rapid Propagation of Stem Buds of Anthurium andraeanum

以红掌嫩茎为外植体,诱导侧芽萌发,并进行增殖和生根培养,研究不同生长调节剂浓度配比对茎段侧芽萌发、增殖、无菌苗生根的影响以及增殖培养过程中愈伤组织的抑制等因素。结果表明,侧芽诱导的适宜培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,萌发率达87.5%;最适增殖培养基为MS+6-BA 0.8 mg/L+NAA 0.2 mg/L+VB2 8.0 mg/L,增殖系数3.8;最适生根培养基为1/2MS+NAA 0.5 mg/L,生根率98%;在增殖培养基中添加适量VB2能较好地抑制愈伤组织的生成,防止愈伤组分织分化形成芽,从而达到以芽繁芽的目的。

The tender stem of Anthurium andraeanum was used as explants to study the effects of different growth regulators concentrations on the stem bud＇s germination, proliferation, rooting culture and the prevention of callus during proliferation culture. The results indicated that the optimal culture medium for stem bud inducement was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5 mg/L, with the germination rate was 87.5%; the optimal culture medium for proliferation was MS ＋ 6-BA0.8 mg/L ＋ NAA 0.2 mg/L ＋ VBz 8.0 mg/L, with the multiplication coefficient was 3.8; the optimal culture medium for rooting was 1/2MS ＋ NAA0.5 mg/L, with the rooting rate was 98%; in addition, appropriate VB2 in proliferation medium could efficaciously prevent the form and differentiation of callus, thus reaching the goal that propagating buds by buds.