木薯酒精废液中不溶性物质厌氧生物水解性能的初步研究

Preliminary Study on the Anaerobic Biological Hydrolysis of Insoluble Compounds in Cassava Stillage

通过分批实验研究了60℃条件下不同pH值对木薯酒精蒸馏废液厌氧水解效果及水解酶活力的影响。考察了不同pH值下的纤维素酶（CMC酶）、半纤维素酶（木聚糖酶）以及游离态和胞上结合态酶活力、水解率、悬浮性固体（SS）降解率、挥发酸（VFA）和乙醇产量以及纤维素和半纤维素降解率。结果表明：维持pH在7.0~8.0时能够获得较高的纤维素酶活力和半纤维素酶活力（1 588和822 IU/L）、SS降解率（37.3%）、纤维素和半纤维素的降解率（54.9%和58.4%）。在此条件下,游离态酶和胞上结合态酶一样,VFA和乙醇总量达到12.6 g/L,且其主要成分为乙酸。

The aim of this manuscript is to determine the effect of pH on the cassava stillage hydrolyzation at 60℃ through compared with the values of the cellulase（CMCase）,hemicellulase（xylase） activity,the free and cell-bound enzyme activities,hydrolytic ratio,the degradation ratio of suspended solids（SS）,the total concentration of volatile fatty acids（VFA） and ethanol,the degradation ratio of cellulose and hemicelluloses at different pH conditions.The results showed that higher cellulase and hemicellulase activity（1 588 and 822 IU/L）,degradation ratio of SS（37.3%）,hydrolytic ratio of cellulose and hemicellulose（54.9% and 58.4%） were obtained when pH kept at 7.0~8.0.Under this condition,the activities of the free and cell-bound enzyme were the same,the total concentration of VFA and ethanol was achieved at 12.6 g/L,where acetate was the main component.