乳腺癌组织TLR4蛋白表达及与细胞凋亡和增殖的关系

Expression of TLR4 and its relationship with cell apoptosis and proliferation in breast cancer

目的探讨乳腺癌组织脂多糖受体(TLR4)的表达及其与细胞凋亡和增殖的关系。方法应用免疫组化法检测乳腺癌及相应癌旁组织TLR4、增殖细胞核抗原(PCNA)的表达,TUNEL法检测组织中细胞的凋亡。结果与癌旁组织比较,乳腺癌组织TLR4表达显著升高(P<0.05)。随着乳腺癌患者T分期、N分期、M分期和细胞凋亡增殖比的增加,乳腺癌组织TLR4的表达升高(P均<0.05),乳腺癌组织TLR4表达与癌组织分化程度无关。结论乳腺癌组织TLR4高表达,促进癌细胞的增殖,在乳腺癌的形成过程中起到了重要的作用。

Objective To investigate the expression of TLR4 in breast cancer and their relationship with cell apoptosis and proliferation. Methods The expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method. Results The TLR4 expression in cancer tissues was significantly higher than that in adjacent tissues. Expression of TLR4 increased gradually with the progress of T stages, N stages, M stages and the ratio of apoptic index to proliferative index （P〈0.05）, but it had no correlation with histological grade. Conclusion TLR4 over expressed in breast cancer can promote cell proliferation, and play an important role in the pathogenesis of human breast cancer.