乙酰葛根素对大鼠脑缺血再灌注VEGF表达的影响

The Effect of Acetylpuerarin on Apoptosis of Neuronal Cell and on the Expression of VGEF after Cerebral Ischemia and Reperfusion Rats

目的探讨脑缺血再灌注后乙酰葛根素的脑保护作用及其机制。方法 线栓法制作脑缺血再灌注模型,分别对假手术组、脑缺血再灌注组、葛根素组、乙酰葛根素低(10mg·kg-1)、中(50mg·kg-1)、高剂量(250mg·kg-1)干预组实验标本作HE染色,采用免疫组化方法观察测定各组大鼠脑内的VEGF的表达情况并进行神经功能缺损评分。结果 脑缺血再灌注组与假手术组比较,VEGF表达增多(P<0.01),药物干预组较缺血再灌注组VEGF表达均增多(P<0.01),且神经元损伤较轻,且三个剂量的表达有显著差别(P<0.01)。结论 乙酰葛根素对脑缺血再灌注有明显的保护作用,诱导VEGF表达可能是其对脑缺血再灌注损伤起保护作用的机制之一。

Objective To investigate the protective effect of acetylpuerarin on cerebral ischemia-reperfusion injury in rats and itsmechanism. Methods Wistar rat was used as a model of focal cerebral ischemia and reperfusion to observe expression of VEGF after focal cerebral ischemia-reperfusion and the protective function of three levels of doses Acetylpuerarin. Immunohistochemicalmethod were used to measure the number of VEGF positive cells in rat’s cerebral cortex of sham-operated group,model group and Acetylpuerarin group. Results VEGF had a high-level expression and increased significantly after ischemia reperfusion（P0.01）.The Acetylpuerarin can significantly increase the expression of the VEGF,and the highest expression after the highest Acetylpuerarin group （P0.01）.Conclusion The Acetylpuerarin had obviously protective function for ischemia-reperfusion rat.