β-连环蛋白和腺瘤样的结肠息肉病蛋白在宫颈上皮内瘤样变及宫颈鳞癌中的表达及意义

Expression and significance of β-catenin and APC in CIN and cervical squamous cell carcinoma

目的探讨β-连环蛋白(β-catenin)和腺瘤样的结肠息肉病(APC)蛋白在宫颈上皮内瘤样变及宫颈鳞癌中的表达及意义。方法采用免疫组化二步法检测β-catenin和APC蛋白在30例宫颈鳞癌、50例宫颈上皮内瘤样变及24例正常宫颈组织中的表达。结果①β-catenin和APC蛋白阳性表达率在正常宫颈组织,宫颈上皮内瘤样变及宫颈浸润鳞癌3组间均有显著差异(P<0.05)。②β-catenin和APC蛋白阴性表达率在CINⅠ、CINⅡ、CINⅢ三组之间比较无统计学差异(P>0.05),β-catenin在CINⅠ组与CINⅡ-CINⅢ组二者之间差异有统计学意义(P<0.05)。③β-catenin阴性表达与APC蛋白阴性表达呈显著正相关(P<0.05)。结论联合检测β-catenin与APC蛋白的表达情况可能有助于宫颈癌的诊断和治疗。

Objective To study the expression and role of β-catenin and APC in cervical squamous cell carcinoma and cervical intraepithelial neoplasia.Methods The expression of β-catenin and APC protein was detected using immunohistochemistry in 30 cases of cervical squamous cell carcinoma,50 cases of cervical intraepithelial neoplasia,and 24 cases of normal cervix tissue.Results ①The abnormal expression of β-catenin and APC in normal cervical mucosa,CIN and cervical squamous cell carcinoma among the three groups were significantly different（P0.05）.②The positive rate of β-catenin and APC among CIN1,CIN2,CIN3 was not statistically significant（P0.05）.But the difference ofβ-catenin between low-level CIN and high-level CIN is significant（P0.05）.③A significantly positive relationship was observed between β-catenin and APC protein expression in cervical carcinoma（P0.05）.Conclusion β-catenin protein and APC protein are appeared in the abnormal expression in the CIN and cervical carcinoma.They play a certain role in the cervical carcinoma occurrence and development.