啤酒糖酵母菌6-磷酸海藻糖合成酶编码基因tps1的cDNA克隆

MOLECULAR CLONING OF tps1 GENE ENCODING TREHALOSE 6 PHOSPHATE SYNTHASE FROM Saccharomyces cerevisiae

本工作从抗逆性极强的啤酒糖酵母菌株 Ａ Ｓ２１４１６ 中分离纯化总 Ｒ Ｎ Ａ 和 ｍ Ｒ Ｎ Ａ ，以 Ａ Ｍ Ｖ 逆转录酶合成了单链ｃ Ｄ Ｎ Ａ 。采用保守引物扩增并克隆了该酵母菌的６ － 磷酸海藻糖合成酶编码基因ｔｐｓ１ ，建立了该基因 Ｄ Ｎ Ａ 片段的物理图谱，发现其酶切位点与已见报道的６ － 磷酸海藻糖合成酶编码基因相同。

Total RNA and mRNA were isolated and purified from yeast. The first strand of cDNA using oligo(dT) as primer was synthesized and a 1 5 kb DNA fragment was obtained with PCR technique. The restriction map of the DNA fragment was analysed. The results showed that the entire cDNA encoding trehalose 6 phosphate synthase had been cloned.\;