Production of Transgenic Pig Clone Embryo Expressed the Red Fluorescent Protein by Using the Somatic Cell Nucleus Transplantation Technology

利用体细胞核移植技术生产表达红色荧光蛋白的猪转基因克隆胚胎(英文)

[Objective] The research aimed to lay the foundation for producing the transgenic clone pig.[Method] The pig fetus fibroblast with the red fluorescent protein（RFP）gene that was transfected by the retrovirus was as the donor of nucleus transplantation.By using the somatic cell cloning technology,the development situation in vitro of clone embryo with RFP was studied.[Result] The fusion rate of RFP transgenic cell was 83.87% which had no significant difference with 80.56% of non-transgenic cell（P0.05）.The blastula rate in vitro of RFP transgenic somatic cell reconstructed embryo was 8.67% which had no significant difference with 6.56% of non-transgenic cell（P0.05）.After the reconstructed embryo of RFP transgenic somatic cell was transplanted into fifteen receptors,there was no conception individual.[Conclusion] The transgenic cell with the red fluorescent protein as the donor could successfully clone the transgenic embryo and obtain the transgenic blastula.

[目的]为生产转基因克隆猪奠定基础。[方法]以反转录病毒转染的带有红色荧光蛋白(RFP)基因的猪胎儿成纤维细胞作为核移植的核供体,利用体细胞克隆技术研究红色荧光蛋白克隆胚胎体外发育情况。[结果]RFP转基因细胞的融合率为83.87%,与未转基因细胸(80.56%)相比无显差异著(P>0.05);RFP转基因体细胞重构胚体外囊胚率为8.67%,与未转基因细胞组(6.56%)相比无显差异著(P>0.05);RFP转基因体细胞重构胚移植于15头受体后,尚无受孕个体。[结论]利用转红色荧光蛋白基因的细胞为供体细胞,并成功地克隆出转基因胚胎。