小麦atpC基因植物超量表达载体的构建

Construction of plant overexpression vector of AtpC gene

线粒体ATP合成酶是氧化磷酸化过程中ATP合成起关键作用的多亚基复合体,但近来发现它们在植物应对非生物胁迫反应中起着十分重要的作用。因此,对ATP合成酶的各亚基基因功能的研究有助于阐释其在植物逆境条件下的调节机制,并为研究植物抗逆和防卫反应提供新的途径。线粒体ATP合成酶是能量代谢的关键酶,参与氧化磷酸化反应。atpC基因所编码的线粒体ATP合成酶γ亚基是线粒体ATP合成酶的功能亚基。为了进一步研究它在胁迫反应中的功能,以小麦cDNA为模板,通过RT-PCR方法扩增出atpC基因。将该基因的cDNA编码序列连接到pCAMBIA1301中,成功构建了小麦pCAMBIA-atpC植物超量表达载体,为后续的转基因研究奠定了基础。

Mitochondrial F1F0-ATP synthase is commonly found in plant and plays a important role in the oxidative phosphorylation process.It also be found recently that plays a great part in plants abiotic stress responses.So study on the function of he subunits of ATP synthase will be helpful to elucidate its regulation and response under diverse stresses,and shows promise for obtaining new ways to understand plant stress and defence responses.Mitochondrial F1F0-ATP synthase is a key enzyme in plant metabolism.The γ subunit of Mitochondrial F1F0-ATP synthase encoded by atpC gene is a functional subunit.In order to study on functions of atpC under stresses,atpC gene was amplified using wheat cDNA as a template.Then a plant overexpression vector of atpC gene was constructed and named pCAMBIA-atpC.This work laid the foundations for future transgenic research.