摘要
用5种蛋白酶(木瓜蛋白酶、中性蛋白酶、typsin、pepsin以及Alcalase 2.4L)分别酶解金枪鱼头蛋白,以羟基自由基清除率为指标,筛选出羟基自由基清除力最强的是Alcalase 2.4L的酶解液,通过响应面试验优化Alcalase2.4L最佳酶解条件为:酶解时间340 min,酶解温度54℃,加酶量0.38%,该条件下酶解液的羟基自由基清除率最佳,为63.67%。
Tuna head protein is hydrolyzed with various proteases（papain,Neutrase,trypsin,pepsin and Alcalase 2.4L） and the hydrolysates are assessed using methods of hydroxyl radical scavenging ability.Hydrolysate prepared with alcalase 2.4L is found to have the highest antioxidant activity.Then the response surface analysis is designed to optimize the hydrolysis conditions of alcalase 2.4L on tuna head protein.And the results show that the optimum hydrolytic conditions is 340 min（time）,54 ℃（temperature）,0.38%（enzyme concentration）,which produces enzymatic hsydrolysate with scavenging ability on hydroxyl free radical of 63.67%.
出处
《农产品加工（下）》
2011年第5期10-14,18,共6页
Farm Products Processing
基金
广东海洋大学自然科学研究资助项目(2009)
关键词
金枪鱼头
酶解
抗氧化
羟基自由基
tuna head
enzymatic hydrolysis
antioxidant
hydroxyl radical
