摘要
[Objective] The aim was to explore the effect of tomato＇s resistance to Botrytis cinerea induced by exogenous Chitosan.[Method] The leaf spraying method was used to determine the induced resistance effect of Chitosan to tomato B.cinerea and the chlorophyll content,soluble protein,soluble sugar,proline influence,the activities of peroxidase（POD）,malondialdehyde（MDA）and the Proline content in leaves of tomato.[Result] The induced resistance of Chitosan reached 58.26% at 14th d after treatment;Compared with the B.cinerea stress group,the chlorophyll content in leaves of tomato seedlings of B.cinerea stress ＋ Chitosan group had increased by 34.63%,while that of soluble protein content,soluble sugar content,Pro content and POD activity was 5.30%,10.83%,16.21% and 16.88%,respectively（except for the MDA content,which was decreased by 16.54%）.[Conclusion] Exogenous Chitosan could improve photosynthetic efficiency and protect enzymes activity to improve the resistance of tomato seedlings to B.cinerea.
[目的]为了明确壳聚糖对番番茄茄幼苗抗灰霉病的诱导作用。[方法]采用叶面喷施的方法,测定了壳聚糖对番茄灰霉病诱抗效果以及番茄叶片中叶绿素含量、可溶性蛋白质、可溶性糖、愈创木酚过氧化物酶(POD)、丙二醛(MDA)、脯氨酸的影响。[结果]壳聚糖对番茄灰霉病的诱抗效果14d时达到58.26%;与灰霉病菌处理组相比,壳聚糖处理组番茄幼苗叶片中叶绿素含量最高增加34.63%、可溶性蛋白含量增加5.30%、可溶性糖含量增加10.83%、游离脯氨酸含量增加16.21%、POD含量增加16.88%、MDA含量降低16.54%。[结论]外源壳聚糖可以提高番茄幼苗光合利用率和保护酶活性从而提高番茄幼苗对灰霉病的抗性。
