摘要
[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of(P.× Citrosum Vanleenii).[Method] By studying the influence of plant growth regulators(PGRs)on explant type(leaves and petioles),the optimal combinations of PGRs to maximize SELSs(somatic embryo-like structure)and buds were established.[Result]0.2 mg/L NAA+1.0 mg/L BA was best for LS(leaves segments)and 0.2 mg/L NAA + 1.5 mg/L BAs was best for PS(petioles segments).Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii.
[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii（P.× Citrosum Vanleenii）is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of（P.× Citrosum Vanleenii）.[Method] By studying the influence of plant growth regulators（PGRs）on explant type（leaves and petioles）,the optimal combinations of PGRs to maximize SELSs（somatic embryo-like structure）and buds were established.[Result]0.2 mg/L NAA＋1.0 mg/L BA was best for LS（leaves segments）and 0.2 mg/L NAA ＋ 1.5 mg/L BAs was best for PS（petioles segments）.Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii.
基金
Supported by Fund of Jinhua Science Technology Foundation of China(2009-2-02)
