Microsatellite Analysis of Genetic Diversity of Large and Middle-sized Donkey Breeds in China

中国大、中型驴种遗传多样性的微卫星分析(英文)

[Objective] The paper aimed to reveal the genetic diversity of large and medium-sized donkey breeds of China.[Method] The genetic polymorphisms of 24 microsatellites in the eight large and medium-sized donkey breeds were studied by means of PCR,polyacrylamide gel electrophoresis and silver staining.[Result] The microsatellite of NVHEQ18 was medium polymorphic and the other 23 microsatellite loci were highly polymorphic.The average PIC（0.694 0）,H（0.711 9）and E（2.9）of the eight donkey breed populations were all high,indicating that the gene polymorphisms and genetic diversity were relatively high.[Conclusion] The 24 microsatellite loci were effective markers for analysis of genetic relationships among the donkey breeds.The phylogenetic relationships among large and medium-sized donkey breeds were in accordance with their breeding history and distribution.

[目的]为分析我国大、中型驴品种遗传多样性。[方法]利用24个微卫星标记,对我国8个大、中型驴品种遗传多样性进行了检测。[结果]24个微卫星位点在8个驴品种中的多态信息含量除NVHEQ18为中度多态外,其余23个微卫星均为高度多态,8个驴种的平均PIC(0.6940)、H(0.7119)和E(3.94),表明基因多态性和遗传多样性相对较高。[结论]这24个微卫星可作为有效的遗传标记用于各驴品种的遗传多样性和系统发生关系的分析。我国大、中型驴品种的分子系统发生关系与其育成史和地理分布基本一致。