乐胺磷对5龄期家蚕肠液蛋白酶活性的影响

Effect of methamidophos-dimethoate emulsifiable on the protease activity of Silkworm midgut

乐胺磷是一种兼有触杀作用和胃毒作用的有机磷广谱杀虫剂,在虫害严重的桑田治理中起到较好作用。但由于乐胺磷的作用对象也包括家蚕在内,用药后乐胺磷在桑叶上的残留对家蚕生理生化的影响仍是一个值得探讨的问题。家蚕肠液蛋白酶活性是检测家蚕消化能力的一个标志性酶。本文用紫外吸收法检测了残留量乐胺磷对5龄期家蚕肠液蛋白酶活性的影响。结果显示,在所添食的乐胺磷浓度范围内,乐胺磷添食量与家蚕肠液蛋白酶活性之间呈负相关。在家蚕5龄初期添食乐胺磷引起的肠液蛋白酶活性的下降,至5龄末期已基本消除。

The protease activity of midgut of silkworm is a mark enzyme of digestive ability of silkworm. Methamidophos-dimethoate emulsifiable concentrate is a contact insecticide and a stomach poison. This paper deals with the technology of effects of methamidophos-dimethoate on the protease activity of midgut of the 5th iinstar lar-va through ultraviolet spectrophotometry. The results showed that the protease activity of midgut of silkworm had negative interaction on concentration of methamidophos-dimethoate.The toxication of methamidophos-dimethoate could cause the distinct decrease of the protease activity at the earlier 5th stage. Both protease activity was a close at the later 5th stage.