摘要
目的了解浙江省台州地区肺炎克雷伯菌ESBLs和AmpCβ-内酰胺酶产生情况及AmpC酶的耐药基因型别特征。方法采用VITEK-AMS60全自动细菌鉴定仪鉴定细菌,按CLSI推荐的确证试验检测ESBLs,采用头孢西丁纸片扩散法筛选疑产AmpC酶阳性菌株,通过酶粗提物三维试验确证产AmpC酶,并经PCR测序等实验分析该菌株的基因型。结果在对头孢西丁不敏感的86株肺炎克雷伯菌中,ESBLs和AmpC酶检出率分别为88.37%和77.91%;以同产ESBLs和AmpC酶为主要形式,占47.67%,单产ESBLs和单产AmpC酶分别占40.70%和30.23%;AmpC酶阳性菌株的耐药基因型:62株为DHA-1型、5株为ACT-1型。结论台州地区肺炎克雷伯菌产ESBLs和AmpC酶检出率较高,AmpC酶以DHA-1型为主。
Objective To understand the producing of ESBLs and AmpC β-lactamase of Klebsiella pneumoniae and the drug-resistant phenotype characteristics of AmpC enzyme in Taizhou.Methods Bacteria were identified with VITEK-AMS60.ESBLs were detected with the confirmatory test recommended by CLSI.Positive strains harboring AmpC enzyme were screened with cefoxitin disk diffusion.Methods AmpC enzyme was confirmed by a three-dimensional test and genotype was differentiated by PCR-sequencing.Results In the 86 isolates of Klebsiella pneumoniae insensitive to cefoxitin,the detection rates of ESBLs and AmpC enzyme were 88.37% and 77.91% respectively.Among them,47.67% produced both ESBLs and AmpC enzyme,40.70% produced only ESBLs and 30.23% produced only AmpC enzyme.The drug-resistant genotypes for AmpC-positive strains were DHA-1（62 isolates） and ACT-1（5 isolates）.Conclusion The detection rate for Klebsiella pneumoniae haboring ESBLs and AmpC enzyme was high in Taizhou and AmpC enzyme mainly belonged to DHA-1 genotype.
出处
《疾病监测》
CAS
2011年第5期380-382,共3页
Disease Surveillance
基金
台州学院培育基金(No.2010PY40)
