转基因水稻对水稻齿叶矮缩病毒(RRSV)抗性评价

Evaluation of resistance of transgenic rice to rice ragged stunt virus (RRSV):

采用人工繁殖带毒褐飞虱（ Ｎｅｌａｐａｒｖａｔａ Ｌｕｇｅｎｓ） 攻毒接种，结合症状观察和ＲＴＰＣＲ 检测方法，对５７ 个含有水稻齿叶矮缩病毒（ＲＲＳＶ） Ｓ７ ，Ｓ９ 和Ｓ１０ 基因片段的转基因水稻对水稻齿叶矮缩病毒的抗性进行了评价。总体而言，大多数转基因稻发病率比受体品种的发病率要低。从中初步得到对ＲＲＳＶ 具有较强抗性的水稻转基因系３１５ 和３１６ ，其发病率分别为１５ ．８ ％ 和１３ ．３ ％ ；具有中等抗性的水稻转基因系１８４ 和３２８ ，发病率分别为２６ ．９ ％ 和２８ ．０ ％ ；而对照受体品种和当地感病品种的发病率则分别为１００ ％ 和８４ ．２ ％ 。将ＲＴＰＣＲ 的方法用于转基因稻抗性的检测，可提高检测的灵敏度。

Fifty seven transgenic rice were evaluated for their resistance to rice ragged stunt virus (RRSV) S7,S9 and S10,through inoculation of RRSV with viruliferous insect vector ( Nelaparvata Lugens) raised in the green house,by combined symptom observation and RT PCR detection.Generally,the infection (%) of most transgenic rice is lower than that of the recipient variety.Transgenic lines 315 and 316 exhibitted strong resistant to RRSV with the infection (%) of 15.8% and 13.3% respectively;Transgneic lines 184 and 328 were also showed middle resistant to RRSV with the infection (%) of 26.9% and 28.0%,respectively.In the mean time,the infection (%) of the recipient variety and the local susceptible variety were 100% and 84.2% respectively.The results indicated that RT PCR method increased sensitivity of RRSV detection in the transgenic rice.