Oct-4在非小细胞肺癌组织中的表达及意义

Expression and significance of Oct-4 in non-small cell lung cancer

目的分析Oct-4在非小细胞肺癌(NSCLC)组织中的表达特点及规律,探讨Oct-4的表达在NSCLC发生、发展中的意义及其与肿瘤干细胞的关系。方法采用免疫组化SP法检测Oct-4蛋白在135例NSCLC组织和20例正常对照肺组织中的表达情况。结果 NSCLC组织中Oct-4表达的阳性率明显高于正常对照肺组织,二者之间的差异具有显著性(P<0.05)。Oct-4表达的阳性率与NSCLC患者的临床分期、有无淋巴结转移及肿瘤分化程度有关(P<0.01),而与患者的性别、年龄及NSCLC的组织学类型无关(P>0.05)。结论 Oct-4的表达与NSCLC的分化程度、临床分期和淋巴结转移有关,提示Oct-4的表达在NSCLC发生、发展中的可能具有一定的意义,表达Oct-4的肿瘤细胞很可能是肿瘤干细胞,推测Oct-4有可能成为分离和鉴定NSCLC肿瘤干细胞的标志物。

Objective To analyze the expression of Oct--4 in non--small lung cancer （NSCLC）, explore its significance in the formation and development of NSCLC and demonstrate its relationship with cancer stem cells. Methods Immunohistochemical detection of Oct--4 was performed in 135 cases of NSCLC and 20 cases of normal lung tissue. Results The expression of Oct--4 was significantly different between NSCLC and normal lung tissue （P 〈 0.05）. The expression of Oct--4 was related with clinical stage, lymph node metastasis and tumor pathological stage （P 〈 0.01）, but was not related with sex, age and histological classification （P 〉0.05）. Conclusions The expression of Oct--4 was related with clinical stage, lymph node metastasis and tumor pathological stage; it suggested that the expression of Oct--4 might be associated with the formation and development of NSCLC. Cells which expressed Oct-- 4 might be the tumor stem cells and Oct--4 might he the marker to isolation and identification of cancer stem cells in NSCLC.