左旋多巴诱导PC12细胞凋亡的实验研究

Experimental research on the apoptosis of PC12 cells induced by L-dopa

目的探讨左旋多巴（L-dopa）治疗帕金森病（PD）疗效减退的机制。方法以PC12（大鼠肾上腺嗜铬细胞瘤）细胞为多巴胺（DA）神经元的细胞模型，应用流式细胞术（FCM）、DNA琼脂糖凝胶电泳、透射电镜、碘化丙啶（propidiumiodide，PI）／HO33342双染色法观察L-dopa对PC12细胞凋亡的影响，每组样本数为7。结果对20μmol／LL-dopa处理组的凋亡率2．5％与对照组2．3％比较无显著差异（P＞0．05），但50、100、150μmol／L的L-dopa作用24小时所致的凋亡率分别为12．4％、24．4％、37．2％，与对照组比较差异有非常显著意义（P＜0．01）。结论在一定浓度范围内的L-dopa可以诱导PC12细胞凋亡，提示凋亡可能参与了L-dopa治疗PD疗效减退的病理过程。

Objective To explore the mechanism of the decline in effectiveness during the treatment ofParkinson's disease with L-dopa. Methods In this study, we used flow cytometry、agarose gel electrophoresis,electron microscopy and PI/HO33324 double staining to detect the induction of apoptosis of the catecholaminergicPC12 cells ny L-dopa. Results The ratios of apoptosis induced by L-dopa at the mranges of the contents of 50,100and 150 μmol/L were 12. 4%, 24.4%, 37. 2% respectively; significantly higher than those of the control group(P<0.01), while the ratio of apoptosis induced by L-dopa at the concentration of 20μmol/L was similar withthat of the controls (P>0. 05).Conclusion The results suggested that apoptosis might be involved in thepathogenetic phase, when the effectiveness of the treatment with L-dopa was declining.