鼠光感受器细胞层移植的方法及观察

A study on transplantation of rat photoreceptor cell layer

目的 研究视锥和视杆细胞与周围组织间在解剖结构及病理生理等方面的关系。方法 取２０ 只同龄Ｗｉｓｔａｒ 和ＲＣＳ鼠作为供体和受体，利用视网膜切削技术取得视锥及视杆细胞层，并植入ＲＣＳ鼠眼视网膜下。术后２ 周时摘除术眼，于光镜下观察并摄影。结果 术后２ 周，在视网膜完全复位的受体眼，移植的纯视锥和视杆细胞成活，可见移植细胞位于视网膜下腔的视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ ，ＲＰＥ）及内颗粒层之间，并与正常对照的视细胞呈相同的染色特点。结论 成片视网膜移殖所制备的动物模型，其视锥和视杆细胞具有正常的解剖位置并与ＲＰＥ及内颗粒层组织相接，为进一步研究重建视神经间的解剖学联系及视神经的生理活动，提供了一种新的途径。

Objective To study the relationship between photoreceptors and other layers of the retina in the aspects of anatomical structure, physiology, pathology, etc . Methods RCS rats were used as host animals, and the pure cone and rod layer of the donor retina was prepared from Wistar rat and was transplanted under the host retina where the cones and rods were degenerated Results After 2 weeks of transplantation, the retinas of the host rats were reattached, and the transplanted cells were survival. The transplanted cells were in the subretinal space between the retinal pigment epithelium (RPE) and the inner unclear layer, and they had the staining characteristics similar to that of the normal control photoreceptos. Conclusion In the animal model of photoreceptor cell layer transplantation, the transplanted cells possess normal anatomical location and are connected with RPE and inner nuclear layer. The results provide a new route to investigate the re establishment of anatomical association with the optic nerve and the physiological activity of the nerve.