不同百草枯中毒剂量对兔肺损伤程度的影响

Effects of different paraquat toxic doses on acute lung injury in rabbits

目的研究不同百草枯(PQ)中毒剂量对兔中毒性急性肺损伤(ALI)及肺纤维化(PF)程度的影响。方法将36只成年新西兰大白兔随机分成3组(A、B、C组),每组12只,分别经胃管灌入8mg/kg(A组)、16mg/kg(B组)和32mg/kg(C组)不同剂量的PQ。7d后处死动物,取肺组织行HE染色、ALI评分并行Masson染色以鉴定PF程度。结果 A组ALI评分为(8.33±1.03)分,明显低于B组([9.83±1.17)分,P=0.047)]和C组([11.50±1.38)分,P<0.01],B组较C组差异也有统计学意义(P=0.03);A组PF程度为(31.09±2.05)%,明显低于B组([34.37±1.62)%,P=0.002]和C组([36.54±0.44)%,P<0.01],B组较C组差异也有统计学意义(P=0.026)。Pearson相关分析显示不同剂量的PQ与ALI、PF程度明显相关。结论随中毒剂量的增大,兔急性肺损伤及PF的病理改变越显著。PQ剂量与肺损伤程度及PF程度密切相关。

Objective To study the effect of different paraquat（PQ） toxic doses on acute lung injury（ALI） and pulmonary fibrosis（PF） in rabbits. Methods Thirty-six adult New Zealand rabbits were randomly divided into three groups according to different doses of paraquat given via grastric canal：group A（8 mg/kg,n=12） ,group B（16 mg/kg,n=12） and group C（32 mg/kg,n=12） . The animals were sacrificed after 7 d poisoning. The upper lobe of lung was removed for HE and Masson staining. The ALI score and PF condition were analyzed and compared. Results The ALI score in Group A [（8.33±1.03） points] were higher than group B [（9.83±1.17） points,P=0.047] and group C [（11.50±1.38） points,P0.01]. The difference between group B and C also showed statically significant（P=0.03） . The PF degree in group A（31.09±2.05） % was lower than group B [（34.37±1.62） %,P=0.002] and group C [（36.54±0.44） %,P0.01]. There is also markedly difference between group B and C（P=0.026） . Pearson correlation analysis showed that different doses of PQ were significantly related with ALI and PF. Conclusions With the toxic dose increasing,the rabbit ALT and the pathological changes of PF become more apparent. PQ doses were closely correlated with ALI and PF.