摘要
目的探讨抗氧化剂普罗布考对2型糖尿病大鼠胰岛细胞氧化应激和胰岛分泌功能的影响。方法选择6周龄雄性Wistar大鼠24只,随机分为3组,每组8只:正常对照组,糖尿病组和普罗布考组。采用高脂饲料喂养加小剂量链脲佐菌素腹腔注射的方法建立2型糖尿病大鼠模型。普罗布考组给予普罗布考500mg/(kg·d),早晚各1次,灌胃1个月。应用葡萄糖氧化酶法﹑酶终点法﹑放射免疫法连续多次检测大鼠空腹血糖(FBG)和餐后2h血糖(PBG)、甘油三酯(TG)、总胆固醇(T-ch)以及空腹胰岛素(FINS)水平,胰腺组织匀浆检测丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)含量。应用免疫组化法检测胰岛素表达变化。结果与正常对照组比较,糖尿病组大鼠胰腺匀浆MDA、NO含量升高,SOD、GSH-Px含量降低,胰岛素表达明显降低,差异均有统计学意义(P均<0.05);与糖尿病组比较,普罗布考组大鼠胰腺匀浆MDA、NO含量降低,SOD、GSH-Px含量升高,胰岛素表达升高,胰岛素分泌功能明显增强(P均<0.05)。结论抗氧化剂可提高2型糖尿病大鼠胰腺对抗氧化应激能力,增强2型糖尿病大鼠胰岛细胞内分泌功能。
Objective To investigate the effect of antioxidant probucol on oxidative stress and secretory function of islet in type 2 diabetes rats. Methods Twenty-four rats were randomly divided into three groups:normal control group(group A) ,diabetic group(group B) and probucol-treated diabetic group(group C) ,8 in each. Rats in group C were treated with probucol for one month [500 mg/(kg/d) ,twice per day]. High-fat and high-sugar diets with a low dose injection of streptozotocin were performed in rats to establish type 2 diabetic models. The methods of glucose oxidase,enzymatic end-point and radioimmunoassay were repeatedly used to test the levels of fasting blood glucose(FBG) ,postprandial blood glucose(PBG) ,triglyceride(TG) ,total cholesterol(T-ch) and fasting insulin(FINS) . Pancreatic tissues were homogenized to test the content of malondialdehyde(MDA) ,nitric oxide(NO) ,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) . Immunohistochemical method was used to detect the insulin expression. Results Compared with rats in the group A,the levels of MDA,NO and FINS were higher,while the levels of SOD,GSH-Px and insulin expression were lower in the group B(all P0.05) . After treated with probucol,compared with the group B,the levels of MDA,NO were lower,while the levels of SOD,GSH-Px and insulin expression were higher in the group C(all P0.05) . Conclusion Probucol can preserve pancreatic islet against damage from oxidative stress and improve the endocrine function of islet cells in type 2 diabetic rats.
出处
《中华危重症医学杂志(电子版)》
CAS
2010年第6期7-10,共4页
Chinese Journal of Critical Care Medicine:Electronic Edition
基金
广西自然科学基金(0542083)
关键词
2型糖尿病
氧化应激
普罗布考
胰岛素
Type 2 diabetes mellitus Oxidative stress Probucol Insulin