胸苷激酶1检测对乳腺癌辅助诊断及化疗效果评估的意义

Predictive value of serum/cytoplasmic thymidine kinase 1 level in the diagnosis and prognosis of breast cancer

目的：探讨血清及细胞质中胸苷激酶1检测对乳腺癌辅助诊断及术后化疗效果评估的意义。方法：2010年1月~2010年12月收治的乳腺癌住院患者共72例,作为实验组,选取42例良性病变患者为对照组。两组同时进行组织TK1（免疫组织化学方法）和血清S-TK1（化学发光法）检测分析。结果：与对照组相比,实验组血清中TK1水平明显升高（P〈0.05）。与对照组相比,实验组细胞质中TK1阳性率明显升高（P〈0.05）。结论：连续跟踪血清及细胞质中TKI水平变化有利于对乳腺癌治疗效果的辅助诊断,其反映细胞增殖情况并与病灶大小有一定相关性,对监视乳腺癌疗效具有临床意义。

Objective： To explore the predictive value of serum/cytoplasmic thymidine kinase 1（TK1） level in the diagnosis and prognosis of breast cancer.Methods： Seventy-two patients with breast cancer that were admitted to our hospital during September 2010~March 2011 were enrolled into the experiment group,and 42 patients with benign diseases as the control group.Serum/cytoplasmic thymidine kinase 1 levels were detected with TK1（immunohistochemistry） and serum S-TK1（chemiluminescence immunoassay,CIA） methods.Results： Both serum and cytoplasmic TK1 levels were significantly elevated comparing with the control group（P0.05）.Conclusions： Serum/cytosolic TKI levels is related to the cellular proliferation status and the size of tumor in patients with breast cancer indicating consecutive detection of TKI levels can help to evaluate the therapeutic effects of anticancer therapy in breast cancer patients.