CD44v6与结直肠癌浸润和转移关系的研究

Relationship between CD44v6 expression and the infiltration and metastasis of colorectal carcinoma

目的:探讨结直肠癌中CD44v6蛋白表达与浸润和转移的关系。方法:采用免疫组织化学链酶亲和素-生物素技术(S-P法)、流式细胞术(FCM)、蛋白印迹技术(Western-blot)检测68例结直肠癌、20例结直肠腺瘤和20例正常结直肠黏膜组织中CD44v6的表达。结果:结直肠癌组织中CD44v6的表达显著高于结直肠腺瘤和正常结直肠黏膜组织(P<0.05),CD44v6的表达与结直肠癌的浸润程度、淋巴结转移和不同Dukes分期相关(P<0.05)。结论:CD44v6的表达与结直肠癌的浸润和转移密切相关,检测CD44v6的表达可作为结直肠癌预后的可靠指标。

Objective： To explore the relationship between CD44v6 expression and the infiltration and metastasis of colorectal carcinoma.Methods： Expression of CD44v6 in colorectal tissues collected from 68 patients with colorectal carcinoma,20 patients with colorectal adenoma and 20 healthy volunteers were detected with immunohistochemical technique,FCM and Western-blot.Results：Expression of CD44v6 in patients with colorectal carcinoma was significant higher than that in their colorectal adenoma and healthy counterparts（P0.05）.Expression of CD44v6 was correlated with the infiltration,metastasis and Dukes stage（P0.05）.Conclusions： CD44v6 was closely related with infiltration,metastasis of colorectal carcinoma and has predictive value in the prognosis of colorectal carcinoma.