摘要
目的:探讨结直肠癌中CD44v6蛋白表达与浸润和转移的关系。方法:采用免疫组织化学链酶亲和素-生物素技术(S-P法)、流式细胞术(FCM)、蛋白印迹技术(Western-blot)检测68例结直肠癌、20例结直肠腺瘤和20例正常结直肠黏膜组织中CD44v6的表达。结果:结直肠癌组织中CD44v6的表达显著高于结直肠腺瘤和正常结直肠黏膜组织(P<0.05),CD44v6的表达与结直肠癌的浸润程度、淋巴结转移和不同Dukes分期相关(P<0.05)。结论:CD44v6的表达与结直肠癌的浸润和转移密切相关,检测CD44v6的表达可作为结直肠癌预后的可靠指标。
Objective: To explore the relationship between CD44v6 expression and the infiltration and metastasis of colorectal carcinoma.Methods: Expression of CD44v6 in colorectal tissues collected from 68 patients with colorectal carcinoma,20 patients with colorectal adenoma and 20 healthy volunteers were detected with immunohistochemical technique,FCM and Western-blot.Results:Expression of CD44v6 in patients with colorectal carcinoma was significant higher than that in their colorectal adenoma and healthy counterparts(P0.05).Expression of CD44v6 was correlated with the infiltration,metastasis and Dukes stage(P0.05).Conclusions: CD44v6 was closely related with infiltration,metastasis of colorectal carcinoma and has predictive value in the prognosis of colorectal carcinoma.
出处
《海南医学院学报》
CAS
2011年第6期731-734,737,共5页
Journal of Hainan Medical University
基金
海南医学院科研基金资助学报项目(0020110184)~~
关键词
CD44V6
结直肠癌
浸润和转移
CD44v6
Colorectal carcinoma
Infiltration and metastasis