硝普钠灌注对移植小肠粘膜细胞凋亡的影响

Effects of Sodium Nitroprusside on Mucosal Epithelium Apoptosis in Rats after Small Bowel Transplantation

目的:探讨外源性一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)对移植小肠粘膜细胞凋亡的影响。方法:64只220～300g雄性SD大鼠随机分成3组:A1组(n=8),仅行剖腹关腹手术;A2组(n=12):12对大鼠随机作为供受体行同种异体节段小肠移植,无SNP干预;A3组(n=16):16对大鼠随机作为供受体行同种异体节段小肠移植,SNP加入灌注液进行供肠灌注。采用前述3组动物模型再灌注5小时肠造口标本,TUNEL法检测小肠蜡块标本的细胞凋亡情况。结果:与A1组(3.86±4.74%)相比,A2(22.44±10.94%)、A3组(17.12±8.44%)小肠粘膜的细胞凋亡指数均有显著增高(P<0.05),A3组较A2组细胞凋亡指数显著降低(P<0.05)。结论:小肠移植导致小肠粘膜细胞凋亡增加,外源性NO供体SNP灌注能够显著降低植入小肠的细胞凋亡,从而可能减弱粘膜屏障的损伤。

Objective： To investigate the effect of sodium nitroprusside （SNP） treatment on mucosal epithelium apoptosis of the transplanted intestine in rat. Methods： Sixty-four male Sprague-Dawley （SD） rats weighing 220-300g were randomly divided into three groups. Group A1 （n=8）： only performed laparotomy. Group A2 （n=12）： 24 rats were randomly assigned to be donor or recipient to establish small bowel transplantation （SBT） model, but no ShIP treatment. Group A3 （n=16）, 32 rats were randomly assigned to be donor or recipient to establish SBT model, flushed with SNP when harvesting the graft. Harvested intestine samples from stoma 5h after reperfusion. Detected apoptosis of transplanted intestine by TUNEL method. Results： There is higher apoptosis index of mucosal epithelial cells of transplanted intestine in group A2（22.44±10.94%） and A3（17.12± 8.44%） than that in A1（3.86± 4.74%） （P〈0.05）. Fewer cells apoptosis was found in group A3 （SNP flushed group） compared with A2. Conclusion： SNP used as a flushing solution element may attenuate the mucosal barrier injury of transplanted intestine by depressing the mucosal cells apoptosis significantly.