摘要
目的:研究东亚钳蝎毒素Martentoxin对脐静脉内皮细胞释放NO的影响。方法:人脐带经胶原酶消化分离后获得脐静脉内皮细胞,经抗血管性血友病8因子鉴定后,用TNF-α建立NO释放模型,观察Martentoxin对脐静脉内皮细胞释放NO的影响。结果:①经6,12,24h作用后,10μM、100μM Martentoxin能降低TNF-α引起的NO释放增加(P<0.05)。②Martentoxin降低TNF-α引起的iNOS活性增强并对TNF-α引起的内皮源性一氧化氮合酶mRNA下调具有一定保护作用(P<0.05)。结论:Martentoxin抑制TNF-α引起的脐静脉内皮细胞释放NO增加。
Objective： To investigate the impact of Martentoxin on Human umbilical vein endothelial cells （HUVECs） releasing nitric oxide induced by TNF-α. Methods： HUVECs were isolated and identified by tmrnunocytochemistry with Anti-von willebrand Factor （vWF）. The NO releasing model of HUVECs was established by treating with 10 ng/ml TNF-α and the effect of Martentoxin on NO releasing was observed. Results： ①Application of 10μM and 100μM Martentoxin inhibits TNF-α-induced NO release after exposure for 6 h, 12 h and 24 h （P〈0.05）. ② Martentoxin inhibits the activity ofiNOS and retarded the down-regulation of eNOS mRNA regulated by TNF-α （P〈0.05）. Conclusion： Martentoxin inhibits the NO production in HUVECs induced by TNF-α.
出处
《现代生物医学进展》
CAS
2011年第11期2045-2048,共4页
Progress in Modern Biomedicine
