摘要
目的:构建CGTHW-1/pGenesil-1-CD151shRNA稳转细胞系和空载体细胞系CGTHW-1/pGenesil-1,探讨CD151对人甲状腺癌CGTHW-1细胞迁移及侵袭的影响及其作用机制。方法:应用Lipofectamine2000将真核干扰载体pGenesil-1-CD 151 shRNA和空载体pGenesil-1导入甲状腺滤泡癌细胞CGTHW-1,经卡那霉素抗性筛选得到稳定的克隆并扩大培养成细胞系,Western blot检测CD151在CGTHW-1细胞中的表达;划痕实验和Transwell实验分别观察CD151对细胞迁移和侵袭能力的影响。结果:成功建立了CGTHW-1/pGenesil-1-CD151shRNA稳转细胞系和空载体细胞系CGTHW-1/pGenesil-1,Western blot结果显示CD151在CGTHW-1细胞中表达,干扰CD151基因后,CGTHW-1细胞的迁移和侵袭能力明显降低。结论:将CD151基因干扰后可明显抑制甲状腺癌细胞的迁移和侵袭能力。CD151可能是影响甲状腺癌侵袭转移的重要因子之一。
Objective： To construct the vector of CGTHW-1/pGenesil-1- CD 151 shRNA for interference and to investigate the impact of CD 151 on the migration and invasion of CGTHW-1 cell in thyroid cancer. Methods： The eukaryotic interference plasmid pGenesil-1-CD151shRNA, and empty vector pGenesil-1 were transfected into CGTHW-1 cell. The cells were selected by kanamycin. After infection, CD151 expression in CGTHW-1 cells was observed by Western blot. Cell migration and invasion were detected by Wound Healing assay and Transwell assay. Results： CGTHW- 1/pGenesil- 1 - CD 151 shRNA and CGTHW- 1/pGenesil- 1 cells were succ- essfully set up. Western blot results showed that CD 151 expression in CGTHW-1 cells. Wound Healing assay and Transwell assay showed that CD 151 gene interfered, CGTHW-1 cells significantly reduced migration and invasion. Conclusion： The interference of CD 151 gene can significantly inhibit the migration and invasion of thyroid cancer cells. CD 151 is likely one of the important factors to affect invasion and metastasis of thyroid cancer.
出处
《现代生物医学进展》
CAS
2011年第11期2071-2074,共4页
Progress in Modern Biomedicine
基金
湘潭市科技计划项目(JZ200704)
