一种改良宫颈鳞癌原代培养方法的建立

Establishment of Modified Method for Primary Culture of Uterine Cervix Cancinoma

目的:探讨不同培养条件对宫颈鳞癌原代培养的可行性。方法:取确诊为宫颈鳞癌患者的活检或手术标本,通过对消化时间及重悬液量进行改良原代细胞培养,观察鳞癌细胞生长状况,并比较其与传统消化法的细胞生长率,免疫组化进行细胞鉴定。结果:消化时间为3小时,重悬液量为1ml时细胞生长率最高。改良法24小时细胞生长率为61.54%,传统消化法24小时细胞生长率为7.69%(x2=5.14,P<0.05)。活检标本细胞生长率80%,手术标本3例无一例细胞生长。结论:采用消化时间为3小时,重悬液量为1 ml成功建立了宫颈鳞癌原代培养方法,活检标本较手术标本更易培养成功。

Objective： To study the possibility of modified method for primary culture of uterine cervix cancinoma. Method： Cervix cancinoma specimens were obtained from patients underwent biopsy or operation, then the primary culture were performed which differ from traditional method in digestion time and resuspension fluid quantity. The cell growth rate were compared between modified and traditional method, also the cell were identified by immunocytochemistry. Results： At 3 hours of digestion time and 1 ml of resuspension fluid, the higher cell growth rate can achieved in modified method （61.54 %）, which was statistical significance when compared with traditional method （7.69 %; x2=-5.14, P〈0.05）. However, either of the two method, no cell growth was observed in operative specimens. Conclusions： A modified method of primary culture in cervix carcinoma can be established at 3 hours of digestion time and 1 ml resuspension fluid quantity, especially for biopsy specimenss.