P2X3受体对中脑导水管周围灰质神经元放电的促进作用

The effect of P2X3 receptors for an increase in firing rate of midbrain periaqueductal gray neurons in vitro

目的：应用脑片膜片钳记录技术，探讨腺嘌呤核苷酸离子通道型3（P2X3）受体活化对大鼠中脑导水管周围灰质（PAG）外侧区（LPAG）神经元膜电位及动作电位发放频率的影响。方法：制备大鼠PAG脑片，在膜片钳全细胞记录模式下，观察LPAG神经元膜电位及动作电位发放频率以及给予P2X3受体激动剂n、β-me三磷酸腺苷（ATP）后对LPAG神经元兴奋性的影响。结果：记录32个LPAG神经元中有29个神经元观察到膜电位升高以及动作电位频率的增加（P〈O．05）。结论：P2X3受体活化具有促进LPAG神经元膜电位升高以及动作电位发放频率增加的作用。

Objective：To investigate the effect of P2X3 receptors activation on membrane potential and action potential firing rate of the neonatal rat lateral midbrain periaqueductac gray（LPAG）neurons. Methods：The neurons of Sprague-Dawley（SD） rats were selected from LPAG slices. The a,β-meATP, a P2X3 receptor agonist, induced alternations of membrane potential and action potential firing rate in these neurons were studied by the whole cell patch clamp technique. Results：The increase of membrane potential and action potential evoked by a,β-meATP were observed in 29 of the 32 recorded LPAG neurons （P 〈0.05）. Conclusion：The activation of P2X3 receptors in the LPAG can increase neuronal membrane potential and action potential firing rate.